Application of genetic engineering methods
- Primer design and cloning using PCR
- Cloning in E. coli and K. phaffii
Fermentation of E. coli, S. sp., K. phaffii and various ascomycetes
- Small scale (1 mL)
- Laboratory scale up to 20 L
- Recombinant protein synthesis
Application of protein-chemical methods
- Fluorescence spectroscopy
- Circular dichroism spectroscopy
- Microscale thermophoresis
Purification of proteins using FPLC
- Immobilized metal affinity chromatography (IMAC)
- Anion exchange chromatography (AEC)
- Size exclusion chromatography (SEC)
Application of bioanalytical methods
- Tricine-/SDS-PAGE and native PAGE
- Western blot and dot blot
Univariate and multivariate data analysis
- Python
- R Studio
Flow cytometry
- Measurement of the metabolic activity of yeasts
- Determination of yeast cell age
- Quantification of mixed fermentations
- Cell cycle analysis
Particle and protein size measurement
- DLS (Dynamic Light Scattering)
- SLS (Static light scattering)
- FT-IR (Fourier-Transform Infrared Spectroscopy)
Synthesis and functionalization of magnetic nanoparticles and surfaces
- Coating of quartz glass surfaces with EDTA
- Silanization of particles and surfaces with subsequent immobilization
Microscopy
- Scanning Electron Microscopy (SEM)
- Confocal Laser Scanning Microscopy (CLSM)