Monography
Advances in Microfluidic Extracellular Vesicle Technology
Book chapter in Advances in Clinical Chemistry
The chapter reviews microfluidic approaches for the isolation, enrichment, and analysis of extracellular vesicles, with a focus on their growing relevance for clinical diagnostics.
DOI: 10.1016/bs.acc.2026.03.004
Application of Filtration in Fruit Juice Processing
Book chapter in Mechanical Separation Processes in the Food Industry. The chapter examines the role of filtration in fruit juice processing, focusing on the removal of particulate matter and microorganisms while preserving the juice’s sensory qualities. It covers mechanical filtration alongside microfiltration, ultrafiltration, and nanofiltration, each suited to specific juice properties, and compares crossflow and dead-end configurations for their impact on clarity, flavor retention, and microbial safety. Particular attention is given to selecting filtration media that avoid nutrient loss, as well as quality assurance measures addressing oxidation and allergen control.
DOI: 10.1016/C2019-0-00066-0
Effect of the singular cell age on Saccharomyces pastorianus ssp. carlsbergensis proliferation
The cumulative dissertation investigates yeast cell aging by using chitin-containing bud scars as surface markers to determine the age of individual cells within populations. By developing a protein linker and employing flow cytometry, bud scars could be non-invasively mapped and used for age-based separation within yeast cultures, allowing for more targeted analysis and control of the fermentation process. Additionally, the study explored the separation of yeast cell populations using magnetic nanoparticles, enabling selective separation and thereby more accurate observation of growth rates depending on cell age under various culture conditions.
Peer-reviewed publications
Replicative age differentially predicts metabolic competence across industrial yeasts at single-cell resolution
Phage-Functionalized Magnetic Separation for Rapid and Selective Detection of Escherichia coli
The study presents carboxymethyl-dextran-coated magnetic nanoparticle clusters functionalized with lytic T4 bacteriophages for the selective capture of Escherichia coli. Particles were synthesized by coprecipitation and characterized by dynamic light scattering, FT-IR spectroscopy, and electron microscopy. Phages were covalently immobilized via EDC/Sulfo-NHS chemistry, and the resulting particles achieved magnetic separation efficiencies of approximately 20 to 40 percent. Rather than introducing a new detection platform, the work provides a mechanistic assessment of the factors governing phage-mediated magnetic separation, identifying key limitations for future system optimization.
Cellular variability as a driver for bioprocess innovation and optimization
Cellular heterogeneity plays a crucial role in biotechnology, impacting metabolic activity, product yield, and process stability. This review explores how factors like asymmetric division, aging, and environmental conditions contribute to functional diversity in microbial populations, particularly yeast. Novel detection and separation techniques, including surface marker-based and label-free approaches, help manage this variability. Emerging technologies such as real-time single-cell analysis and microfluidics offer promising solutions for optimizing industrial bioprocesses, enhancing yield, and ensuring product quality.
Exploring Multi-Parameter Effects on Iron Oxide Nanoparticle Synthesis by SAXS Analysis
This study shows how adjusting factors like temperature and mixing speed can make iron oxide nanoparticles just the right size for medical uses like MRI and drug delivery. We found that higher temperatures and specific stirring speeds significantly impact final particle size and particle growth, helping fine-tune their properties. These insights make it easier to create nanoparticles that work better in real-life medical settings.
My Congress presentations
2026
Connecting replicative age and cellular vitality in heterogeneous yeast populations
VH Yeast Conference 2026, Berlin
2024
Age matters: Yeast cell fractionation for optimizing bioprocesses and bioproducts
1st International Scientific Conference »Pharmacy of the Future: From Research to Market«, Tuzla
Professional Publications
Brauwelt (2024)
Altersabhängige Sortierung von Saccharomyces mit millifluidischen Systemen
Eigenfeld, M.; Schwaminger, S.P.; Kerpes, R.; Becker, T.
Brauwelt (2024)
Bewertung der Messmethoden für die Viabilitätsbestimmung von Hefe
Eigenfeld, M.; Wittmann, L.; Schwaminger, S.P.; Kerpes, R.; Becker, T.